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The sealed kit consists of:
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Test membrane enclosed in a plastic cassette.
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A small plastic pipette for dispensing the urine sample.
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Desiccant pack to maximize the shelf-life of the test kit
A detailed instruction sheet is provided with the test kit.
FoalProof Equine offers rapid and accurate tests for diagnosing the pregnancy status of mares from 120 days after breeding through to the last 6 weeks of the expected time of foaling, giving you answers in 5-20 minutes.
FoalProof Equine uses lateral flow technology to analyze a small sample urine for the presence of metabolites of pregnancy specific steroids secreted by the developing fetal-placental unit, and thereby aid the diagnosis of pregnancy status. In the event of foetal loss, production of the estrogenic steroid decreases rapidly to non-pregnant levels.
NO FALSE POSITIVES
WeeFoal™ urine test can be used to diagnose and monitor pregnancy status in all breeds of horses, donkeys and zebra, where palpation per rectum or internal ultrasound scanning is not an option for miniature or smaller mares.
NON-INVASIVE, ACCURATE, AND AFFORDABLE
URINE COLLECTION
The WeeFoal test is best performed on freshly collected urine which may be obtained at any time of the day. The test may also be performed on collected urine which has been stored for up to 48 hours at 4 degrees centigrade. It does not have to be in sterile conditions. A wee bit of dirt wont hurt. To see an example of collecting urine for this test, see our on-line example.
TEST PROCEDURE
The WeeFoal test can be performed on a urine sample collected from 120 days after mating through to expected foaling. It is not recommended to use the test before 120 days have elapsed after mating. Run the test at room temperature and in a well-lighted area. The urine sample and test pack should be at room temperature before performing the test. The urine sample must be diluted as described below.
Do not run the test with undiluted urine. When ready to perform the test, open the foil pouch and place the test cassette, 2 plastic pipettes and tube of urine diluent on a flat, clean surface. Take one of the pipettes, squeeze the upper bulb and draw enough of the urine sample into the pipette to fill the stem completely. (0.15 ml will fill the stem, and any overdrawn amount will go into the lower bulb reservoir). Discharge the urine sample from the pipette stem into the tube of urine diluent by gently squeezing the upper bulb (any excess fluid from the draw will remain in the reservoir). Replace the cap on the tube of urine diluent and invert the tube twice to thoroughly mix the urine with the diluent. Take the second pipette, put the stem in the tube of diluted urine, and draw enough of the diluted urine sample into the pipette to fill the stem completely. Discharge the diluted urine sample drop by drop from the pipette stem into the round sample well of the cassette by gently squeezing the upper bulb (any excess fluid from the draw will remain in the reservoir).
Allow the sample to soak into the absorbent pad in the well between drops. Once a few drops have been added to the sample well, the urine will start to flow along the membrane in the cassette window. Continue adding drops of diluted urine until the leading edge of the fluid is ¾ of the way along the membrane, i.e. has reached the ‘C’ mark on the plastic housing.
Do not flood the sample well by adding too much sample, or adding it too quickly.
Wait for 5 to 10 minutes. The test result will generally be obvious within 5 minutes but occasionally it may take up to 10 minutes for the result to clearly form.
Interpreting the result
Positive Negative
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